在线阅读 --自然科学版 2009年3期《高效液相色谱中的蛋白质手性固定相》
高效液相色谱中的蛋白质手性固定相--[在线阅读]
穆惠英1,2, 敦惠娟1, 苗凤智3, 魏雨1
1. 河北师范大学化学与材料科学学院, 河北石家庄 050016;
2. 河北化工医药职业技术学院化学与环境工程系, 河北石家庄 050026;
3. 河北科技大学环境科学与工程学院, 河北石家庄 050018
起止页码: 362--367页
DOI:
摘要
对近年来报道的各种高效液相色谱蛋白质手性固定相的研究进行了综述.主要介绍了蛋白质手性固定相的制备、分类、组成、性质、拆分机理及其应用,讨论了各类蛋白质手性固定相的优缺点,指出了目前存在的问题和今后的研究方向.

Protein-based Chiral Stationary Phase for High-performance Liquid Chromatography Enantioseparations
MU Hui-ying1,2, DUN Hui-juan1, MIAO Feng-zhi3, WEI Yu1
1. College of Chemistry and Material Science, Hebei Normal University, Hebei Shijiazhuang 050016, China;
2. Department of Chemistry and Environmental Engineering, Hebei Chemical andPharmaceutical Vocational Technology College, Hebei Shijiazhuang 050026, China;
3. College of Environmental Science and Engineering, Hebei University of Science & Technology, Hebei Shijiazhuang 050018, China
Abstract:
The enantioseparations of various compounds using proteins as the chiral selectors in high-performance liquid chromatography (HPLC) were considered. The recent development in the classification,the preparation,the properties,the chiral recognition mechanism and the applications of chiral stationary phases used in high performance liquid chromatography were int roduced. The advantages and disadvantages of different chiral stationary phases were also discussed. The problems at present, the research direction and focus for the future have been put forward.

收稿日期: 2008-7-24
基金项目: 河北省自然科学基金(E2006000167);河北省教育厅科学研究项目(2006438)

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