在线阅读 --自然科学版 1998年3期《转CaM基因工程菌的培养及重组钙调素的提纯》
转CaM基因工程菌的培养及重组钙调素的提纯--[在线阅读]
白娟, 刘洪涛, 孙大业
河北师范大学生物系, 050016, 石家庄
起止页码: 389--391页
DOI:
摘要
研究了转植物CaM基因的大肠杆菌的培养以及从该工程菌中分离纯化CaM的方法,观察到在含氨节青霉素及执菌素的LB液体培养基中,在35℃培养至在600nm的光吸收为0.5~0.7后,收集菌体破碎,并经苯基琼脂糖疏水层析柱亲和层析,可得到高纯度的CaM,其最高得率为每升菌液得到20mg的CaM.
关键字: 钙调素 工程菌

The Culture of Transgenic E. coli and Purification of Calmodulin from the Engineering Bacteria
Bai Juan, Liu Hongtao, Sun Daye
Department of Biology, Hebei Normal University, 050016, Shijiazhuang, PRC
Abstract:
The methods for the culture of transgenic E. colt and purification of plant:almodulin from the above engineering bacteria were investigated. It was shown that the3unable density of engineering bacteria as judged by A,,, is from 0. 5-0. 7,as the engineering bacteria was cultured in LB liquid medium containing 100 mg/L kanamycin and25 mg/L chloramphenicol. The calmodulin in the engineering bacteria was purified byphenyl-sepharose 4B affinity chromatography and the purity was judged by SDS PAGE,about 20 mg of purified calmodulin was able to be purified from 1 liter of the engineering bacteria.

收稿日期: 1998-07-09
基金项目: 国家自然科学基金

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