在线阅读 --自然科学版 2000年2期《普通小麦热激处理后育性变化和A、T型CMS品系不育机理的初探》
普通小麦热激处理后育性变化和A、T型CMS品系不育机理的初探--[在线阅读]
葛荣朝, 魏艳菊, 李瑞玲
河北师范大学生命科学学院 河北 石家庄 050016
起止页码: 256--259页
DOI:
摘要
用普通小麦的不育系和保持系为材料,经高温处理以后,提取其线粒体多肽和可溶性蛋白做SDS-PAGE分析,发现不育系与保持系在热激后其线粒体多肽的电泳图谱均有相互趋同的现象.育性不稳定的A型繁91小麦雄性不育系在热激后有明显的可育度提高现象.育性稳定的T型细胞质雄性不育(CytoplasmicMaleSterility,CMS)品系及其保持系经短时间热激后没有明显育性变化.由未经热激处理的对照电泳分析可见,A型不育系、T型CMS品系花粉发育到单核期向双核期转变时,其线粒体多肽较其相应保持系明显减少,表明到此时期不育系细胞的线粒体结构与功能已明显丧失

Study of the Fertility Change of the Wheat after Being Heated Shock and Sterile Principle of A, T Type CMS Lines
GE Rong chao, WEI Yan ju, LI Rui ling
College of Life Science, Hebei Normal University, Shijiazhuang 050016, China
Abstract:
The male sterile lines and their corresponding maintainers of wheat were treated under higher temperature. After that the mitochondrions polypeptides and the dissolved proteins were abstracted and analyzed by SDS-PAGE. It was found that the mitochondrions polypeptides of male sterile lines and corresponding maintainers all tended to the same SDS-PAGE patterns as each others. The unsteady A-type Fan91 male sterile line showed the significant fertility restoring after heating shock. But the steady CMS-T lines and their maintainers didn 't show the changing of fertility after short time 's heating shock. Analyzed the natural control 's SDS-PAGE patterns,it was found that the mitochondrions polypeptides of CMS-T, A-type male sterile lines decreased significantly contrast with their corresponding maintainers. That is, the structure and function of the mitochondrions in male sterile lines 'pollens had lost significantly when the pollens developed from uninuclear period to binuclear period.

收稿日期: 1998-12-15
基金项目:

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