在线阅读 --自然科学版 2000年2期《异细胞质在八倍体小黑麦育种上利用的研究》
异细胞质在八倍体小黑麦育种上利用的研究--[在线阅读]
何聪芬1, 李集临2
1. 河北师范大学生命科学学院 河北 石家庄 050016;
2. 哈尔滨师范大学生物系黑龙江 哈尔滨 150080
起止页码: 252--253页
DOI:
摘要
利用15个异细胞质中国春及对照中国春分别与黑麦杂交,结实率有所不同,出苗率差异显著,异细胞质对F1减数分裂中期染色体配对有影响,Ae.sharonesieAe.bicornis细胞质分别促进和抑制中国春与黑麦F1部分同源染色体配对.用八倍体小黑麦回交F1,回交结实率差异显著,t检验表明,异细胞质对F1产生有功能雌配子有影响,Ae.crassa细胞质诱发雄蕊雌化.继续用八倍体小黑麦回交,得到4种异细胞质八倍体小黑麦,细胞学观察表明:减数分裂不稳定,多价体明显增多.利用Ae.juvenalis,T.timopheevi等4种细胞质可以将黑麦中有益基因直接转移给小麦

Study of the Utilization of Alien Cytoplasm in the Breeding of Octoploid Triticale
HE Cong fen1, LI Ji lin2
1. College of Life Science Biology, Hebei No rmal Univ ersity, Shijiazhuang 050016, China;
2.Department of Biology, HaRBin Normal University, HaRbin 150080,China
Abstract:
15 Wheat variety“ Chinese Spring” with different alien cytoplasms were crossed with rye,and their F1 were crossed with stable octoploid triticale. Small differences in seed-set ratio, significant difference in emergence ratio and plumpness of kernel among different lines of alloplasmic F1, BC1and BC2 were observed. Four lines of alloplasmic octoploid triticale were made in BC 2. Different cytoplasms effects the homologous chromosome pairing as shown by Chiasma frequency in F1 at metaphase of first meiotic division and on producing active female gamete as shown by backcrossing seed-set rate with stable octoploid triticale. The results indicated that transferring of desirable genes from rye to Chinese Spring with timopheevi and jurenalis alloplasmes is feasible. In BC2( alloplasmic octoploid triticale lines) ,one or more univalents were observed in PMCS at metaphaseⅠ of meiosis,indicating their meiosis were unstable.

收稿日期: 1999-12-12
基金项目:

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