在线阅读 --自然科学版 2021年1期《响应曲面法优化复合酶提取林芝绿茶中茶多酚工艺》
响应曲面法优化复合酶提取林芝绿茶中茶多酚工艺--[在线阅读]
郝治华, 李颖, 张唐伟, 吴雪莲, 次仁德吉
西藏自治区农牧科学院 农业质量标准与检测研究所, 西藏 拉萨 850032
起止页码: 53--60页
DOI: 10.13763/j.cnki.jhebnu.nse.202103001
摘要
以西藏林芝茶树老叶为材料,采用复合酶有机溶剂法提取茶叶中茶多酚.在单因素实验的基础上,应用响应曲面法进一步优化提取工艺,得到最佳提取条件:纤维素酶用量6mg,果胶酶用量8mg,溶剂体积分数68%,料液质量浓度0.024g/mL,提取温度56℃,提取时间129min,pH=5.0,茶多酚提取率为15.61%.

Optimization of Polyphenol Extraction from Linzhi Green Tea with Complex Enzymes by Response Surface Methodology
HAO Zhihua, LI Ying, ZHANG Tangwei, WU Xuelian, Cirendeji
Institute of Agricultural Product Quality Standard and Inspection, Tibet Academy of Agricultural and Animal Husbandry Sciences, Tibet Lasa 850032, China
Abstract:
Taking the old leaves of Linzhi tea tree in Tibet as materials,tea polyphenols in tea were extracted by complex enzyme assisted organic solvent method.On the basis of single factor experiment,the extraction process was optimized by response surface method.The optimum extraction conditions are as follows:the dosage of cellulase 6mg,pectinase 8mg,respectively,the volume fraction of solvent is 68%,mass concentratron of material liquid 0.024g/mL,extraction temperature 56℃,extraction time 129min,pH is 5.0.The final extraction rate of tea polyphenols was 15.61%.

收稿日期: 2019-12-19
基金项目: 西藏自治区自然科学青年基金(2018ZRG-39)

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