在线阅读 --自然科学版 2017年5期《泥河湾藉箕滩遗址土壤微生物的分离与系统发育分析》
泥河湾藉箕滩遗址土壤微生物的分离与系统发育分析--[在线阅读]
吕春爽, 崔艳美, 梁思源, 水小溪, 赵亚超, 李忠达, 李越, 赵宝华, 刘东
河北师范大学 生命科学学院, 河北 石家庄 050024
起止页码: 440--447页
DOI: 10.13763/j.cnki.jhebnu.nse.2017.05.013
摘要
采用平板稀释涂布法从泥河湾藉箕滩遗址土壤中分离微生物,结合菌株形态特征、生理生化特征及分子鉴定方法对分离到的微生物进行鉴定,以确定泥河湾藉箕滩遗址不同深度土层中微生物物种分布,为详细了解遗址土壤微生物物种多样性提供依据.结果表明:共分离鉴定到96株细菌、34株放线菌、15株真菌.根据同源性比对及构建系统发育进化树,确定细菌归属于芽孢杆菌属和短波单胞菌属;放线菌归属链霉菌属;真菌归属青霉属、曲霉属、被孢霉属和盐单胞菌藻.NHWB 1047,NHWA 1017,NHWF 1002等菌株与已知菌种相似度低于97%,可能为潜在新种.本研究首次从泥河湾藉箕滩遗址不同深度土层中分离鉴定微生物,是泥河湾考古研究的重要组成部分,为进一步了解史前时代生态环境提供了科学依据.

Separation and Phylogenetic Analysis of Soil Microorganisms in Jijitan Ancient Site of Nihewan Basin
LYU Chunshuang, CUI Yanmei, LIANG Siyuan, SHUI Xiaoxi, ZHAO Yachao, LI Zhongda, LI Yue, ZHAO Baohua, LIU Dong
College of Life Science, Hebei Normal University, Hebei Shijiazhuang 050024, China
Abstract:
In order to determine the distribution of the microbial species in soils from Jijitan ancient site in Nihewan basin,we investigated the species diversity of edaphon in different depths of soil layer.Microbial species were isdated from soil samples by dilution plate method.The pure cultures of microbial species were determined by morphological observation,physiological and biochemical characterization as well as 16SrDNA and 18SrDNA sequencing.96 bacterial strains,34 actinomycetes strains and 15 fungal strains were isolated and identified.Based on the results of homologous analysis of sequences and phylogenetic tree,the strains of bacteria belong to Bacillus sp.and Brevunmdimonas sp.;the strains of actinomycetes belong to Streptomyces sp.;and the strains of fungi belong to Penicillium spp.Aspergillus spp.,Mortierella spp.and Halomonas spp.The similarity of 16SrDNA and 18SrDNA sequences of strains NHWB 1047,NHWA 1017,and NHWF 1002 with known microbial species are less than 97%,suggesting that they may be potential new species.The study is the first time to isolate and identificate the microbial species in different depths of soil layer from Jijitan ancient site in Nihewan basin.The study is an important part of archaeological study of Nihewan,providing scientific data for us to further understand the prehistoric ecological environment.

收稿日期: 2017-03-20
基金项目: 河北省教育厅优秀青年基金(YQ2014026);河北师范大学校内重点基金(L2016Z03)

参考文献:
[1]ROSELLÕ-MORA R,AMANN R.The Species Concept for Prokaryotes[J].FEMS Microbiology Review,2001,25(1):39-67.doi:10.1111/j.1574-6976.2001.tb00571.x
[2]GANS J,WOLINSKY M,DUNBAR J.Computational Improvements Reveal Great Bacterial Diversity and High Metal Toxicity in Soil[J].Science,2005,309(5739):1387-1390.doi:10.1126/science.1112665
[3]贾真秀.泥河湾远古文化探研[J].河北北方学院学报(社会科学版),2011,27(5):72-76.doi:CNKI:13-1354/C.20111009.0953.017
[4]庞其清,翟大有,赵筑簾,等.泥河湾盆地晚新生代微体古生物地层及环境演化的探讨[J].地质学报,2015,89(5):817-842.doi:10.3969/j.issn.0001-5717.2015.05.001
[5]朱日祥,邓成龙,潘永信.泥河湾盆地磁性地层定年与早期人类演化[J].第四纪研究,2007,27(6):922-944.doi:10.3321/j.issn.1001-7410.2007.06.008
[6]同号文,王法岗,郑敏,等.泥河湾盆地新发现的梅氏犀及裴氏板齿犀化石[J].人类学学报,2014,33(3):369-388.doi:10.16359/j.cnki.cn11-1963/q.2014.03.003
[7]DENG T,WANG S Q,HOU S K.A Bizarre Tandemhomed Elasmothere Rhino from the Late Miocene of Northwestern China and Origin of the True Elasmothere[J].Chinese Science Bulletin,2013,58(15):1811-1817.doi:10.1007/s11434-012-5574-4
[8]李君,刘建超,姬凌飞.旧石器遗址中的遗迹——以泥河湾为例[J].文物春秋,2015(6):3-14.doi:10.13635/j.cnki.wwcq.2015.06.001
[9]邢卉研,王继华,魏京焘.哈尔滨师范大学样地土壤微生物的分离与鉴定[J].哈尔滨师范大学自然科学学报,2014,30(3):159-162.doi:10.3969/j.issn.1000-5617.2014.03.043
[10]程娟娟,赵徐平,张利平,等.泥河湾大长梁遗址和马圈沟I文化层放线菌的分离及系统发育分析[J].基因组学与应用生物学,2016,35(4):913-917.doi:10.13417/j.gab.035.000913
[11]夏占峰,关统伟,张利莉,等.艾丁湖沉积物放线菌多样性[J].微生物学报,2011,51(8):1023-1031.doi:10.13343/j.cnki.wsxb.2011.08.015
[12]楼骏,柳勇,李延.高通量测序技术在土壤微生物多样性研究中的研究进展[J].中国农学通报,2014,30(15):256-260.doi:10.11924/j.issn.1000-6850.2013-2513
[13]王燕华,牛平山.河北泥河湾地质遗迹自然保护区的建设与管理[J].河北国土资源,2006(11):11-13.