在线阅读 --自然科学版 2009年1期《MDV中L-meq基因对MDCC-MSB1细胞端粒酶活性影响的研究》
MDV中L-meq基因对MDCC-MSB1细胞端粒酶活性影响的研究--[在线阅读]
郑梅竹1, 赵骥民2, 潘风光3, 时东方1, 刘春明1
1. 长春师范学院中心实验室, 吉林长春 130032;
2. 长春师范学院生命科学学院, 吉林长春 130032;
3. 吉林大学军需科技学院, 吉林长春 130062
起止页码: 89--93页
DOI:
摘要
构建了真核表达载体pcDNA3.1(+)-L-meq,利用脂质体2000转染MDCC-MSB1细胞,采用改进的端粒重复序列扩增程序(telomere repeat amplification protocol,TRAP)法、实时荧光定量RT-PCR方法检测转染前后端粒酶活性以及端粒酶反转录酶(telomerase reverse transcriptase,chTERT)chTERT mRNA的表达量的变化,探讨L-meq基因对细胞端粒酶活性的影响.结果表明L-meq基因在MDCC-MSB1细胞中能够稳定表达,L-MEQ的稳定表达能够下调chTERTmRNA的表达水平,并抑制了MDCC-MSB1细胞的端粒酶活性,使其相对端粒酶活力下降.

Study on the Effect of L-meq Gene of MDV on the Telomerase Activity in MDCC-MSB1 Cell
ZHENG Mei-zhu1, ZHAO Ji-min2, PAN Feng-guang3, SHI Dong-fang1, LIU Chun-ming1
1. The Central Laboratory, Changchun Normal University, Jilin Changchun 130032, China;
2. College of Life Science, Changchun Normal University, Jilin Changchun 130032, China;
3. College of Light Industry and Economics, Jilin University, Jilin Changchun 130062, China
Abstract:
To characterize the properties and functions of L-meq gene,recombinant eukaryon expression vector pcDNA3.1(+)-L-meq was const ructed and transfected into MDCC-MSB1,a chicken lymp hoblastoid cell line transformed by Marek's disease virus by liposome 2000.Updated TRAP method and Real-time PCR was used to test the telomerase activity and ch TERT mRNA respectively before and after transfection.The results showed that,the cells transfected with pcDNA3.1(+)-L-meq stably expression L-M EQ protein even until 96 hours after transfection.The L-meq protein can suppress the expression of telomerase activity,softly down with the relative telomerase activity and also can depress the expression level of ch TERT.It is reasonable to presume that L-meq can be a transrepressor which suppress the telomerase activity by L-meq protein.

收稿日期: 2008-6-8
基金项目: 国家自然科学基金(30471284)

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