在线阅读 --自然科学版 2016年6期《毛细管电泳间接检测法分析树叶中的重金属离子》
毛细管电泳间接检测法分析树叶中的重金属离子--[在线阅读]
韩康1, 刘二虎2, 韩亚楠1, 翟学良1
1. 河北师范大学 分析测试中心, 河北 石家庄 050024;
2. 香港奥星生命科技, 河北 石家庄 050011
起止页码: 519--522页
DOI: 10.13763/j.cnki.jhebnu.nse.2016.06.011
摘要
采用弱络合剂乙醇酸,间接检测毛细管区带电泳法(CZE)对重金属离子Ba2+,Cr3+,Cd2+,Pb2+,Ni2+进行了分离研究.通过对背景电解质种类和浓度、弱络合剂的浓度、缓冲液pH、微波消解条件等的优化,确定了以12mmol/L乙醇酸-10mmol/L吡啶(pH=3.8)为电泳缓冲液,采用压力进样方式,在10kV恒压下对树叶中的重金属离子进行分离,并在波长255nm处检测.5种重金属离子标样在1.0~30.0mg/L呈良好线性关系,3个水平的加标回收率为93.5%~100.6%.

Heavy Metal Ions in Leaves by Capillary Electrophoresis with Indirect Detection
HAN Kang1, LIU Erhu2, HAN Yanan1, ZHAI Xueliang1
1. Instrumental Analysis Center, Hebei Normal University, Hebei Shijiazhuang 050024, China;
2. Hongkong Aoxing Life Science and Technology, Hebei Shijiazhuang 050011, China
Abstract:
Capillary zone electrophoresis with on-column complexation using glycolic acid,indirect UV detection was applied to the separation of Ba2+,Cr3+,Cd2+,Pb2+ and Ni2+ from leaves.The influence of carrier electrolyte's kind and concentration,glycolic acid concentration and pH,as well as conditions of microwave assisted digestion,were investigated and discussed.The heavy metal ions were separated in a 12mmol/L glycolic acid-10mmol/L pyridine(pH=3.8) buffer,applied voltage of 10kV and detection wavelength of 255 nm.Under the optimum conditions,the calibration curves of five heavy metal ions were linear in the range of concentrations 0.1~30mg/L.The recovery of the method was 93.5%~100.6%.

收稿日期: 2015-4-13
基金项目: 河北师范大学青年科学基金(L2013Q11)

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