在线阅读 --自然科学版 2015年4期《毛竹硅转运基因 PhLsi1 的 ORF克隆及生物信息学分析》
毛竹硅转运基因 PhLsi1 的 ORF克隆及生物信息学分析--[在线阅读]
马欢1,2, 王凯2, 吴妙丹3, 赵宝华1
1. 河北师范大学 生命科学学院, 河北 石家庄 050024;
2. 中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室, 浙江 杭州 310006;
3. 浙江农林大学 林木与生物技术学院, 浙江 杭州 311300
起止页码: 345--351页
DOI: 10.13763/j.cnki.jhebnu.nse.2015.04.012
摘要
以水稻硅的内转运蛋白基因 Lsi1 的cDNA序列为信息探针,对毛竹的核酸数据库进行同源性搜索,获得全长为797bp的毛竹硅内转运蛋白基因的cDNA序列(Genbank登陆号:FP095571).与水稻 Lsi1 的cDNA序列相比,毛竹中该基因cDNA序列第721位处发生终止突变,导致毛竹 Lsi1 丢失了86个氨基酸残基.采用RT PCR方法对其进行克隆,序列分析表明, PhLsi1 基因在编码时确实存在提前终止现象,共编码212个氨基酸,与水稻、玉米和大麦中已克隆的硅内转运蛋白(silicon influx transporter,含有6个跨膜螺旋区和2个NIP保守基序)的序列相似性分别高达99.3%,91.9%,91.9%,但只具有5个跨膜螺旋区和1个NIP保守基序.

ORF Cloning and Bioinformatics Analysis of Silicon Transporter PhLsi1 in Phyllostachys edulis
MA Huan1,2, WANG Kai2, WU Miaodan3, ZHAO Baohua1
1. College of Life Science, Hebei Normal University, Hebei Shijiazhuang 050024, China;
2. Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Zhejiang Hangzhou 310006, China;
3. School of Forestry and Bio-technology, Zhejiang Forestry University, Zhejiang Hangzhou 311300, China
Abstract:
By homologous searching in Phyllostachys edulis nucleotide database with the cDNA of rice silicon influx transporter Lsi1 as probe,we obtained a cDNA sequence (Genbank accession number:FP095571) of P.edulis silicon influx transporter gene with a length of 797 bp.Compared with cDNA of rice Lsi1,a stop codon appeared in advance at the 721st base of its counterpart in P.edulis,which resulted in the deletion of 86 amino acid residues in Lsi 1 of P.edulis (PhLsi 1).The early termination of the gene PhLsi 1 was confirmed by amplification with RT-PCR and sequence analyzing.The PhLsi1 encodes 212 amino acid residues,forming only five transmembrane helices and one NIP conserved domain, which has a similarity of 99.3%,91.9% and 91.9%,respectively, with the silicon influx transporter (comprise of six ransmembrane helices and two NIP conserved domains) already cloned from rice,maize and barly.

收稿日期: 2015-5-9
基金项目: 2008年度中国水稻研究所自主研究课题(ZZKT200801)

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